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α sma primary antibody  (Proteintech)


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    Structured Review

    Proteintech α sma primary antibody
    α Sma Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+sma+primary+antibody/smooth+muscle+actin+Polyclonal+antibody/pm41933369-130-1-9
    Average 96 stars, based on 1237 article reviews
    α sma primary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Quercetin ameliorates epithelial-mesenchymal transition and inflammation by targeting FSTL1 and modulating the NF-κB pathway in pulmonary fibrosis
    Article Snippet: A549 or BEAS-2B cells were inoculated in six-well plates and fixed in 4% paraformaldehyde for 20 min. Paraffin sections were then subjected to dewaxing and antigen repair treatment, followed by infiltration of 0.5% Triton X-100 (9002-93-1, Coolaber, Beijing, China) for 5 min and then closed with 2% bovine serum albumin (BSA) (A8020, Solarbio, Beijing, China) for 45 min. .. The cells were then incubated with FSTL1 (1:1600 dilution, 20182-1-AP, Proteintech, Wuhan, China) and α-SMA primary antibody (1:400 dilution, 67735-1-Ig, Proteintech, Wuhan, China) at 4°C overnight. .. After washing the cells or sections with PBS for 5 min, which was performed three times to remove unbound antibodies, the cells were incubated with CoraLite ® Plus 488-labeled goat anti-rabbit secondary antibody (RGAR002, Proteintech, Wuhan, China) and CoraLite ® Plus 594-labeled goat anti-mouse secondary antibody (RGAM004, Proteintech, Wuhan, China) were incubated at room temperature for 1 h. The nuclei were finally stained with DAPI dye (C1005, Beyotime Biotechnology, Shanghai, China) and finally stained with anti-fade sealer.

    Article Title: Therapeutic effects of IL-33/ST-2 pathway inhibition combined with albendazole on hepatic fibrosis and immune regulation in alveolar echinococcosis: in vivo and in vitro evidence.
    Article Snippet: .. An α-SMA primary antibody (smooth muscle actin-specific recombinant antibody, Proteintech Cat. No. 14395-1-AP, Proteintech, Rosemont, IL, USA) was added and incubated at room temperature for 30 min, followed by incubation with a fluorescent secondary antibody for 20 minutes. α-SMA expression was quantified using a BD FACSCelesta flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). ..

    Article Title: Early growth response 1 exacerbates thoracic aortic aneurysm and dissection of mice by inducing the phenotypic switching of vascular smooth muscle cell through the activation of Krüppel-like factor 5.
    Article Snippet: .. For double immunofluorescence staining, the slides were incubated with Egr1 primary antibody (1:100, ab300449, Abcam, Cambridge, UK) and Alexa Fluor® 488- conjugated goat anti- rabbit IgG (1:500, GB25303, Servicebio, Wuhan, China), and then incubated with α- SMA primary antibody (1:1500, 14 395- 1- AP, Proteintech, Wuhan, China) and Cy3conjugated goat anti- rabbit IgG (1:500, GB21303, Servicebio, Wuhan, China). .. The nuclei were stained with 4,6- diamidino- 2- phenylindole (DAPI) (G1012, Servicebio, Wuhan, China), and images were obtained via a fluorescence microscope.

    Article Title: Study on the effect of Mongolian medicine Qiwei Qinggan Powder on hepatic fibrosis through JAK2/STAT3 pathway.
    Article Snippet: Sections were visualized by a microscope and the ratio of collagen deposition (blue color area) over the whole field area was quantified by ImageJ software (National Institutes of Health, Bethesda, Maryland, USA). .. For Immunohistochemistry, sections were incubated with α-SMA primary antibody (Proteintech Group, Wuhan, Hubei, China) overnight at 4 °C, followed by incubation with secondary antibody (Maixin Biotechnology Development Co., Ltd, Fuzhou, Fujian, China) for 1 h. Finally, the expression of α-SMA was observed under microscope. ..

    Article Title: Salvianolic acid B and sodium tanshinone II A sulfonate prevent pulmonary fibrosis through anti-inflammatory and anti-fibrotic process.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: FXR deficiency in hepatocytes disrupts the bile acid homeostasis and inhibits autophagy to promote liver injury in Schistosoma japonicum -infected mice
    Article Snippet: For western blot assay, the protein was resolved in SDSS gels and transferred to PVDF membrane (1620177, Bio-RAD, California, United States). .. After blocking with 5% milk for 3 h at room temperature, membranes were incubated with FXR primary antibody (25055-1-AP, Proteintech, Wuhan, China), α-SMA primary antibody (14395-1-AP, Proteintech, Wuhan, China), LC3 primary antibody (3868, Cell Signaling Technology, Boston, United States), Beclin-1 primary antibody (11306-1-AP, Proteintech, Chicago, United States), P62 primary antibody (18420-1-AP, Proteintech, Chicago, United States), and GAPDH primary antibody (60004-1-lg, Proteintech, Chicago, United States) at 4°C overnight. ..

    Recombinant:

    Article Title: Therapeutic effects of IL-33/ST-2 pathway inhibition combined with albendazole on hepatic fibrosis and immune regulation in alveolar echinococcosis: in vivo and in vitro evidence.
    Article Snippet: .. An α-SMA primary antibody (smooth muscle actin-specific recombinant antibody, Proteintech Cat. No. 14395-1-AP, Proteintech, Rosemont, IL, USA) was added and incubated at room temperature for 30 min, followed by incubation with a fluorescent secondary antibody for 20 minutes. α-SMA expression was quantified using a BD FACSCelesta flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). ..

    Expressing:

    Article Title: Therapeutic effects of IL-33/ST-2 pathway inhibition combined with albendazole on hepatic fibrosis and immune regulation in alveolar echinococcosis: in vivo and in vitro evidence.
    Article Snippet: .. An α-SMA primary antibody (smooth muscle actin-specific recombinant antibody, Proteintech Cat. No. 14395-1-AP, Proteintech, Rosemont, IL, USA) was added and incubated at room temperature for 30 min, followed by incubation with a fluorescent secondary antibody for 20 minutes. α-SMA expression was quantified using a BD FACSCelesta flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). ..

    Article Title: Study on the effect of Mongolian medicine Qiwei Qinggan Powder on hepatic fibrosis through JAK2/STAT3 pathway.
    Article Snippet: Sections were visualized by a microscope and the ratio of collagen deposition (blue color area) over the whole field area was quantified by ImageJ software (National Institutes of Health, Bethesda, Maryland, USA). .. For Immunohistochemistry, sections were incubated with α-SMA primary antibody (Proteintech Group, Wuhan, Hubei, China) overnight at 4 °C, followed by incubation with secondary antibody (Maixin Biotechnology Development Co., Ltd, Fuzhou, Fujian, China) for 1 h. Finally, the expression of α-SMA was observed under microscope. ..

    Flow Cytometry:

    Article Title: Therapeutic effects of IL-33/ST-2 pathway inhibition combined with albendazole on hepatic fibrosis and immune regulation in alveolar echinococcosis: in vivo and in vitro evidence.
    Article Snippet: .. An α-SMA primary antibody (smooth muscle actin-specific recombinant antibody, Proteintech Cat. No. 14395-1-AP, Proteintech, Rosemont, IL, USA) was added and incubated at room temperature for 30 min, followed by incubation with a fluorescent secondary antibody for 20 minutes. α-SMA expression was quantified using a BD FACSCelesta flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). ..

    Double Immunofluorescence Staining:

    Article Title: Early growth response 1 exacerbates thoracic aortic aneurysm and dissection of mice by inducing the phenotypic switching of vascular smooth muscle cell through the activation of Krüppel-like factor 5.
    Article Snippet: .. For double immunofluorescence staining, the slides were incubated with Egr1 primary antibody (1:100, ab300449, Abcam, Cambridge, UK) and Alexa Fluor® 488- conjugated goat anti- rabbit IgG (1:500, GB25303, Servicebio, Wuhan, China), and then incubated with α- SMA primary antibody (1:1500, 14 395- 1- AP, Proteintech, Wuhan, China) and Cy3conjugated goat anti- rabbit IgG (1:500, GB21303, Servicebio, Wuhan, China). .. The nuclei were stained with 4,6- diamidino- 2- phenylindole (DAPI) (G1012, Servicebio, Wuhan, China), and images were obtained via a fluorescence microscope.

    Staining:

    Article Title: Flavonifractor Plautii or Its Metabolite Desaminotyrosine as Prophylactic Agents for Alleviating Myocardial Ischemia/Reperfusion Injury.
    Article Snippet: .. Briefly, the cells were stained with an α-SMA primary antibody (14395-1-AP, Proteintech, China) and a fluorescent secondary antibody (AS053, Abclonal, China). .. The α-SMA fluorescence intensity was then visualized and quantified using a Cytation 5 fluorescence multiwell plate reader (Agilent, USA).

    Article Title: Flavonifractor Plautii or Its Metabolite Desaminotyrosine as Prophylactic Agents for Alleviating Myocardial Ischemia/Reperfusion Injury
    Article Snippet: .. Briefly, the cells were stained with an α‐SMA primary antibody (14395‐1‐AP, Proteintech, China) and a fluorescent secondary antibody (AS053, Abclonal, China). .. The α‐SMA fluorescence intensity was then visualized and quantified using a Cytation 5 fluorescence multiwell plate reader (Agilent, USA).

    Immunohistochemistry:

    Article Title: Study on the effect of Mongolian medicine Qiwei Qinggan Powder on hepatic fibrosis through JAK2/STAT3 pathway.
    Article Snippet: Sections were visualized by a microscope and the ratio of collagen deposition (blue color area) over the whole field area was quantified by ImageJ software (National Institutes of Health, Bethesda, Maryland, USA). .. For Immunohistochemistry, sections were incubated with α-SMA primary antibody (Proteintech Group, Wuhan, Hubei, China) overnight at 4 °C, followed by incubation with secondary antibody (Maixin Biotechnology Development Co., Ltd, Fuzhou, Fujian, China) for 1 h. Finally, the expression of α-SMA was observed under microscope. ..

    Microscopy:

    Article Title: Study on the effect of Mongolian medicine Qiwei Qinggan Powder on hepatic fibrosis through JAK2/STAT3 pathway.
    Article Snippet: Sections were visualized by a microscope and the ratio of collagen deposition (blue color area) over the whole field area was quantified by ImageJ software (National Institutes of Health, Bethesda, Maryland, USA). .. For Immunohistochemistry, sections were incubated with α-SMA primary antibody (Proteintech Group, Wuhan, Hubei, China) overnight at 4 °C, followed by incubation with secondary antibody (Maixin Biotechnology Development Co., Ltd, Fuzhou, Fujian, China) for 1 h. Finally, the expression of α-SMA was observed under microscope. ..

    Blocking Assay:

    Article Title: FXR deficiency in hepatocytes disrupts the bile acid homeostasis and inhibits autophagy to promote liver injury in Schistosoma japonicum -infected mice
    Article Snippet: For western blot assay, the protein was resolved in SDSS gels and transferred to PVDF membrane (1620177, Bio-RAD, California, United States). .. After blocking with 5% milk for 3 h at room temperature, membranes were incubated with FXR primary antibody (25055-1-AP, Proteintech, Wuhan, China), α-SMA primary antibody (14395-1-AP, Proteintech, Wuhan, China), LC3 primary antibody (3868, Cell Signaling Technology, Boston, United States), Beclin-1 primary antibody (11306-1-AP, Proteintech, Chicago, United States), P62 primary antibody (18420-1-AP, Proteintech, Chicago, United States), and GAPDH primary antibody (60004-1-lg, Proteintech, Chicago, United States) at 4°C overnight. ..



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